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There are two primary forms of B12 used in injections

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Why Deficiency Matters Even mild deficiency can cause wide-ranging symptoms: Persistent fatigue or low energy Brain fog, mood changes, or poor concentration Tingling or numbness in hands and feet Pale skin or shortness of breath Reduced immune function These occur because B12 is critical for oxygen transport and nerve conduction

For silencing FOXO4, specific small interfering RNA (siRNA) was employed, with a negative control siRNA, and transfection was conducted using Lipofectamine 3000 [2]